Mass Spectrometry-Based Lipidomics: Methods and Protocols by Fong-Fu Hsu (Englis

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Mass Spectrometry-Based Lipidomics

by Fong-Fu Hsu

This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature.

FORMAT Hardcover LANGUAGE English CONDITION Brand New

Publisher Description

This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves asan invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

Back Cover

This detailed volume covers conventional MS-based "shotgun lipidomics" by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.

Table of Contents

Mass Spectrometry-Based Lipidomics: An Overview.- High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry.- Global Lipidomics Profiling by a High Resolution LC-MS Platform.- Comprehensive Structural Characterization of Lipids by Coupling Paternò–Büchi Reaction and Tandem Mass Spectrometry.- Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis.- Quantitative Analysis of Polyphosphoinositide, Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun Lipidomics after Methylation.- A Mass Spectrometry-Based Shotgun Lipidomics Using Charge-Switch Derivatization for Analysis of the Complex Long-Chain Fatty Acids.- One-Pot Extractive Transesterification of Fatty Acids Followed by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS.- Ceramide Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole Instrument.- Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High Resolution Mass Spectrometry Platform.- Mass Spectrometric Analysis of Meibomian Gland Lipids.- Quantification of Plasma Oxylipins Using Solid Phase Extraction and Reversed-Phase Liquid Chromatography-Triple Quadrupole Mass Spectrometry.- Targeted Lipidomics of Drosophila melanogaster During Development.- Shotgun Lipidomic Analysis of Leishmania Cells.- Characterization of the Uncommon Lipid Families in Corynebacterium glutamicum by Mass Spectrometry.- Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi.- Analytical Methodologies for Lipidomics in Hemp Plant.- Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry.- Imaging of Polar and Nonpolar Lipids Using Desorption Electrospray Ionization/Post-Photoionization Mass Spectrometry.- Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach.

Feature

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Details ISBN1071614096 Short Title Mass Spectrometry-Based Lipidomics Series Methods in Molecular Biology Language English Year 2021 ISBN-10 1071614096 ISBN-13 9781071614099 Format Hardcover Subtitle Methods and Protocols Series Number 2306 DOI 10.1007/978-1-0716-1410-5 Publisher Springer-Verlag New York Inc. Edition 1st Imprint Springer-Verlag New York Inc. Place of Publication New York, NY Country of Publication United States Pages 314 Publication Date 2021-05-06 AU Release Date 2021-05-06 NZ Release Date 2021-05-06 US Release Date 2021-05-06 UK Release Date 2021-05-06 Author Fong-Fu Hsu Edited by Fong-Fu Hsu Edition Description 1st ed. 2021 Alternative 9781071614129 DEWEY 572.57 Audience Professional & Vocational Illustrations 62 Illustrations, color; 29 Illustrations, black and white; XI, 314 p. 91 illus., 62 illus. in color. With online files/update.

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TheNile_Item_ID:143968885;
  • Condition: Brand New
  • ISBN-13: 9781071614099
  • Book Title: Mass Spectrometry-Based Lipidomics
  • ISBN: 9781071614099
  • Publication Year: 2021
  • Type: Textbook
  • Format: Hardcover
  • Language: English
  • Publication Name: Mass Spectrometry-Based Lipidomics: Methods and Protocols
  • Item Height: 254mm
  • Author: Fong-Fu Hsu
  • Publisher: Springer-Verlag New York Inc.
  • Item Width: 178mm
  • Subject: Chemistry
  • Item Weight: 816g
  • Number of Pages: 314 Pages

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